Preparation and characterization of a purified influenza virus neuraminidase vaccine
Identifieur interne : 001D06 ( Main/Exploration ); précédent : 001D05; suivant : 001D07Preparation and characterization of a purified influenza virus neuraminidase vaccine
Auteurs : Michael Hocart [États-Unis] ; Bernardo Grajower [États-Unis] ; Armen Donabedian [États-Unis] ; Barbara Pokorny [États-Unis] ; Charles Whitaker [États-Unis] ; Edwin D. Kilbourne [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 1995.
English descriptors
- Teeft :
- Affinity chromatography, Antibody response, Antigenic, Antigenic stimulation, Assay, Column volumes, Definitive immunity, Electron micrographs, Elisa test, Enzymatic activity, Experimental animals, Final concentration, Fluorometric assay, Hemagglutinin, Immunogenic, Immunogenic characterization, Immunologic response, Influenza, Influenza virus, Influenza virus neuraminidase, Intravirionic antigenic competition, Johansson, Kilbourne, Molecular weight, Neuraminidase, Neuraminidase vaccine, Rich supernate, Secondary response, Sodium acetate solution, Sodium bicarbonate solution, Tetrameric heads, Total protein, Vaccine, Vibrio cholerae, Viral, Viral hemagglutinin, Virus, Virus preparation, Virus vaccine, Zealand rabbits.
Abstract
Abstract: Influenza virus neuraminidase (NA) has been shown to induce protective but infection-permissive immunity in experimental animals. Challenge infection following such immunization is attended by decreased viral replication and disease manifestations but is sufficient to provide antigenic stimulation and definitive immunity to the virus. The present report describes the preparation and characterization of a purified NA vaccine (NAV) used in Phase 1 (immunogenicity and toxicity) trials in humans. In essence, virion NA was isolated from detergent-disrupted virus by affinity chromatography on oxamic acid-agarose, treated with formalin and tested for its enzymatic activity and for its immunogenicity in Balb c mice and New Zealand rabbits. The preparation was essentially free of viral hemagglutinin but contained residual NP and M1 proteins. Both dispersed and aggregated NA tetrameric heads were seen in electron micrographs. Enzymatic activity was preserved, and minimal immunogenic doses in mice and rabbits, respectively, were 3.7 and 0.027 μg per kg.
Url:
DOI: 10.1016/0264-410X(95)00126-L
Affiliations:
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Kilbourne</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">13</biblScope><biblScope unit="issue">18</biblScope><biblScope unit="page" from="1793">1793</biblScope><biblScope unit="page" to="1798">1798</biblScope></imprint><idno type="ISSN">0264-410X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Affinity chromatography</term><term>Antibody response</term><term>Antigenic</term><term>Antigenic stimulation</term><term>Assay</term><term>Column volumes</term><term>Definitive immunity</term><term>Electron micrographs</term><term>Elisa test</term><term>Enzymatic activity</term><term>Experimental animals</term><term>Final concentration</term><term>Fluorometric assay</term><term>Hemagglutinin</term><term>Immunogenic</term><term>Immunogenic characterization</term><term>Immunologic response</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Intravirionic antigenic competition</term><term>Johansson</term><term>Kilbourne</term><term>Molecular weight</term><term>Neuraminidase</term><term>Neuraminidase vaccine</term><term>Rich supernate</term><term>Secondary response</term><term>Sodium acetate solution</term><term>Sodium bicarbonate solution</term><term>Tetrameric heads</term><term>Total protein</term><term>Vaccine</term><term>Vibrio cholerae</term><term>Viral</term><term>Viral hemagglutinin</term><term>Virus</term><term>Virus preparation</term><term>Virus vaccine</term><term>Zealand rabbits</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Influenza virus neuraminidase (NA) has been shown to induce protective but infection-permissive immunity in experimental animals. Challenge infection following such immunization is attended by decreased viral replication and disease manifestations but is sufficient to provide antigenic stimulation and definitive immunity to the virus. The present report describes the preparation and characterization of a purified NA vaccine (NAV) used in Phase 1 (immunogenicity and toxicity) trials in humans. In essence, virion NA was isolated from detergent-disrupted virus by affinity chromatography on oxamic acid-agarose, treated with formalin and tested for its enzymatic activity and for its immunogenicity in Balb c mice and New Zealand rabbits. The preparation was essentially free of viral hemagglutinin but contained residual NP and M1 proteins. Both dispersed and aggregated NA tetrameric heads were seen in electron micrographs. Enzymatic activity was preserved, and minimal immunogenic doses in mice and rabbits, respectively, were 3.7 and 0.027 μg per kg.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Hocart, Michael" sort="Hocart, Michael" uniqKey="Hocart M" first="Michael" last="Hocart">Michael Hocart</name></region><name sortKey="Donabedian, Armen" sort="Donabedian, Armen" uniqKey="Donabedian A" first="Armen" last="Donabedian">Armen Donabedian</name><name sortKey="Grajower, Bernardo" sort="Grajower, Bernardo" uniqKey="Grajower B" first="Bernardo" last="Grajower">Bernardo Grajower</name><name sortKey="Kilbourne, Edwin D" sort="Kilbourne, Edwin D" uniqKey="Kilbourne E" first="Edwin D." last="Kilbourne">Edwin D. Kilbourne</name><name sortKey="Pokorny, Barbara" sort="Pokorny, Barbara" uniqKey="Pokorny B" first="Barbara" last="Pokorny">Barbara Pokorny</name><name sortKey="Whitaker, Charles" sort="Whitaker, Charles" uniqKey="Whitaker C" first="Charles" last="Whitaker">Charles Whitaker</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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